A fluorescence lifetime estimation method for incomplete decay
نویسندگان
چکیده
A new incomplete decay signal model is proposed to describe the incomplete decay effects in a time-correlated single-photon counting (TCSPC) based fluorescence lifetime imaging (FLIM) system. Based on this model, we modified a MUltiple SIgnal Classification (MUSIC) algorithm to eliminate the incomplete decay effects. Monte Carlo simulations were carried out to demonstrate the performances of the proposed approach. Simulations show that the proposed method is insensitive to the laser pulse rate and has a larger lifetime dynamic range compared with previously reported approaches. As far as we know, this new method is the first non-fitting method that can resolve incomplete decay effects for multi-exponential decays.
منابع مشابه
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Fluorescence lifetime imaging has emerged as an important microscopy technique, where high repetition rate lasers are the primary light sources. As fluorescence lifetime becomes comparable to intervals between consecutive excitation pulses, incomplete fluorescence decay from previous pulses can superimpose onto the subsequent decay measurements. Using a mathematical model, the incomplete decay ...
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(2015) Fluorescence lifetime estimation method for incomplete decay. This document is made available in accordance with publisher policies and may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the URL above for details on accessing the published version. Copyright and all moral rights...
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